Carmine Content: 0.5-1.0% w/v.
Acetic Acid Concentration: 45% v/v ± 2%.
Appearance: Deep red, homogeneous solution without precipitate.
Filterability: Passes through filter paper without residue.
Staining Performance: Produces clear, distinct chromosome staining.
pH: 2.0-2.5.
Solubility: Complete, no undissolved particles.
Shelf Life: 12 months when stored properly.
Microbial Growth: No visible microbial growth.
Precipitation: No precipitation on standing for 24 hours.
Clarity: Clear solution when examined against light.