Dye Content (Spectrophotometry): ≥ 90.0 %
Appearance: Purple to reddish-purple powder.
Solubility: Soluble in water, giving a purple solution.
pH Transition Range: Yellow 5.2 – 6.8 Purple.
Absorbance (0.04% in water):
λmax (acidic form): ~433 nm
λmax (basic form): ~591 nm
A₅₉₁/A₄₃₃ ratio: within specified batch range.
Water Content (Karl Fischer): ≤ 5.0 %
Residue on Ignition: 10.0 – 13.0 % (as sodium salt).
Heavy Metals (as Pb): ≤ 20 ppm
DNase, RNase, Protease Activity: Not detected.
Gel Electrophoresis Suitability: Does not interfere with DNA/RNA migration or staining.
pH Indicator Performance: Clear color change in appropriate buffers.
