Formula: Concentrated nitric acid layered over concentrated sulfuric acid
CAS: Mixture
EC No.: Mixture
M (Molar Mass): Not applicable
D (Density): ~1.4-1.8 g/cm³ (two layers)
mp (Melting Point): Not applicable
bp (Boiling Point): Not applicable
Optical Rotation: Not applicable
Chemicals Properties
Composition:
Concentrated sulfuric acid: Bottom layer
Concentrated nitric acid: Top layer
Ratio: Typically 1:1 by volume
Appearance: Two distinct layers, colorless to pale yellow
Protein Test (Xanthoproteic): With proteins produces yellow color turning orange with alkali
Sensitivity: Detects protein at concentrations ≥ 1 mg/mL
Reaction Time: Color develops within 1-2 minutes
Preparation: Carefully layered to prevent mixing
Hazard Information
Hazard: EXTREMELY CORROSIVE. Causes severe skin burns and eye damage. Reacts violently with many organic materials
Safety: Use only in fume hood with face shield, acid-resistant gloves, and apron. Add acids slowly to prevent splashing
Storage: Store in a cool place in a tightly closed, acid-resistant container (preferably in fume hood). Label clearly as corrosive. Prepare fresh as needed
