Formula: Kit containing Crystal Violet, Gram's Iodine, Decolorizer, and Safranin/Counterstain CAS: Mixture EC No.: Mixture M (Molar Mass): Not applicable D (Density): Various mp (Melting Point): Not applicable bp (Boiling Point): Not applicable Optical Rotation: Not applicable

Components: Crystal Violet Solution: 0.5-1.0% in water/alcohol Gram's Iodine (Lugol's): Iodine 1%, KI 2% in water Decolorizer: Ethanol or acetone-alcohol mixture Counterstain: Safranin 0.25-0.5% or basic fuchsin Appearance: Various colored solutions as per component Performance: Clearly differentiates Gram-positive (purple) and Gram-negative (pink/red) bacteria Volume: Typically 100 mL or 250 mL per component Shelf Life: 1-2 years when stored properly